PlpE epitopes of Pasteurella multocida fusion protein as novel subunit vaccine candidates
نویسندگان
چکیده
منابع مشابه
In silico Analysis of Pasteurella multocida PlpE Protein Epitopes As Novel Subunit Vaccine Candidates
Background: Pasteurella multocida is a Gram-negative, non-motile, non-spore forming, and aerobic/anaerobic cocobacillus known as the causative agent of human and animal diseases. Humans can often be affected by cat scratch or bite, which may lead to soft tissue infections and in rare cases to bacteremia and septicemia. Commercial vaccines against this agent include inactivated, live attenuated,...
متن کاملMolecular heterogeneity of plpE gene in Indian isolates of Pasteurella multocida and expression of recombinant PlpE in vaccine strain of P. multocida serotype B: 2
Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence w...
متن کاملImmunogenicity and protective efficacy of the recombinant Pasteurella lipoprotein E and outer membrane protein H from Pasteurella multocida A:3 in mice.
Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil...
متن کاملIdentification of novel immunogens in Pasteurella multocida
UNLABELLED P. multocida is a Gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. Current vaccines include bacterins, which provide only limited protection against homologous serotypes. Therefore there is a need for more effective vaccines to control diseases caused by P. multocida. As a step towards developing...
متن کاملFormulation of an effective Pasteurella multocida vaccine for sheep.
An effective vaccine for the immunization of sheep against Pasteurella multocida infection was prepared from P. multocida Strain D4 (Type D) and a selected strain of P. multocida Type A. Provided an adequate concentration of bacteria was used, the vaccine thus formulated induced antibodies in sheep that protected mice not only against the vaccine strains but also against infection by a number o...
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ژورنال
عنوان ژورنال: Advanced Biomedical Research
سال: 2020
ISSN: 2277-9175
DOI: 10.4103/abr.abr_245_19